AutoCAL® is a system of laboratory robots and software that facilitates the high-throughput selection and screening of HuCAL antibody libraries resulting in the rapid selection of a highly diverse pool of specifically binding antibody fragments.
Features
- High-throughput automated system
- Modular and expandable
- Reduced reaction volumes in microtiter plates lead to less consumption of antigen
- Inline quality control by integrated controls and multiple measurements
- Extremely low intra- and interassay variability
- Efficient data management
- Output: Specific monoclonal ELISA binders
In order to automate and increase the throughput in antibody generation, the selection process of HuCAL antibodies was simplified, miniaturized and parallelized. The automated system allows the generation of high-quality, validated and sequenced antibodies in a highthroughput fashion against hundreds of different antigens. The modular system of specialized workstations enables parallel and interlaced handling of work packages. An up-scale of capacity is easily attainable by “cloning” the robotic system as it is.
AutoPan® Selection
- Binding of antibody-displaying phage to immobilized antigen
- Removal of unspecifically bound phage by stringent washing
- Amplification of specifically bound phage
- Expression of the antibodies on the surface of phage for the next round of selection
AutoScreen® Screening Steps
- Automated picking of single bacterial colonies each harboring a HuCAL antibody gene and transfer into microtiter plates
- Primary screening of thousands of single clones in a robust 384 well ELISA
- Automated transfer of a pre-defined selection of ELISA-positive clones for further validation
- Test of cross-reactivity and quality control of ELISA-positive clones
- Sequence analysis of validated antibodies and database entry
